Fig. 4. Suppression of Maxi-Cl currents by overexpression of a phospho-mimicking ANXA2 mutant (ANXA2-Y23E) in C127 cells. A, B. The effects of overexpression of wild-type (WT) ANXA2 and ANXA2-Y23E on Maxi-Cl activity. Upper panels show the representative time courses of Maxi-Cl current activation after patch excision from the cells transfected with Anxa2-WT-DsRed2 as the control (A, top panel) and transfected with the phospho-mimicking mutant Anxa2-Y23E-DsRed2 (B, top panel). During the records, alternating pulses from at 0 to ±25 mV were applied. Bottom panels show the voltage-dependent inactivation pattern of Maxi-Cl currents elicited by applying voltage pulses (500 ms) from 0 to ±50 mV in 10-mV increments at the time points indicated by triangles in upper panels. C. Summary of the effects of overexpression of wild-type ANXA2 (WT) and phospho-mimicking ANXA2-Y23E (Y23E) on Maxi-Cl currents recorded at +25 mV. Each column represents the mean ± SEM (vertical bar). *p<0.05 (Student's t-test) vs WT.